| 產(chǎn)品編號(hào) | bsm-61142R |
| 英文名稱 | Musashi 1 Recombinant Rabbit mAb |
| 中文名稱 | 神經(jīng)干細(xì)胞RNA結(jié)合蛋白Musashi1重組兔單抗 |
| 別 名 | RNA-binding protein Musashi homolog 1; Musashi-1; MSI1. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 17A15 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測(cè)分子量 | 39 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Musashi 1: 1-52/362 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. SWISS: O43347 Gene ID: 4440 Musashi1是一種進(jìn)化保守的RNA結(jié)合蛋白，在維持干細(xì)胞狀態(tài)、分化和腫瘤發(fā)生方面起著重要作用。Musashi1 選擇性地表達(dá)在神經(jīng)前體細(xì)胞上,包括神經(jīng)干細(xì)胞上。在神經(jīng)系統(tǒng)外，Musashi1還是腸干細(xì)胞的選擇性標(biāo)記。這些組織干細(xì)胞或未成熟細(xì)胞Musashi1的表達(dá)，表明Musashi1在轉(zhuǎn)錄后基因調(diào)節(jié)階段維持這些細(xì)胞未分化狀態(tài)起重要作用。Musashi1過(guò)度表達(dá)通過(guò)依賴RBP2Jk的旁路激活Notch1，而Notch信號(hào)途徑功能為誘導(dǎo)哺乳動(dòng)物神經(jīng)干細(xì)胞自我更新。通過(guò)musashi1-P-小鼠培養(yǎng)腦細(xì)胞 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Musashi 1 monoclonal antibody, unconjugated (bsm-61142R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Musashi 1? Monoclonal Antibody, Unconjugated(bsm-61142R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Musashi 1? Monoclonal Antibody, Unconjugated(bsm-61142R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Musashi 1) monoclonal Antibody, unconjugated (bsm-61142R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The SH-SY5Y(H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-Musashi 1 antibody (bsm-61142R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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