| 產(chǎn)品編號 | bsm-61148R |
| 英文名稱 | Phospho-AKT1S1 (Thr246) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化蛋白激酶AKT底物1重組兔單抗 |
| 別 名 | AKT1S1; PRAS; PRAS40. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 11F12 |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | 40 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human AKT1S1 around the phosphorylation site of T246: PRLN-pT-SDF 237-249/256 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. SWISS: Q96B36 Gene ID: 84335 |
| 產(chǎn)品圖片 |
NIH/3T3 (M) cells were treated with or without insulin (150nM) for 15 min,Hela (H) cells were treated with or without insulin (100nM) for 5 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-AKT1S1 (Thr246) monoclonal antibody, unconjugated (bsm-61148R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
