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LIN28B Recombinant Rabbit mAb (bsm-61215R)  
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25ul/800.00元
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產(chǎn)品編號(hào) bsm-61215R
英文名稱 LIN28B Recombinant Rabbit mAb
中文名稱 RNA結(jié)合蛋白LIN28B重組兔單抗
別    名 CSDD2; FLJ16517; LIN28B; Protein lin-28 homolog B.  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào)
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:1000-1:2000,Flow-Cyt=1:50-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 27 kDa
檢測分子量 30
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human Lin 28B: 1-250 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA.

SWISS:
Q6ZN17

Gene ID:
389421

產(chǎn)品圖片
Western blot analysis of K562 cell lysate. Using LIN28B (bsm-61215R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-LIN28B antibody (bsm-61215R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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