| 產(chǎn)品編號 | bsm-61275R |
| 英文名稱 | BPI Recombinant Rabbit mAb |
| 中文名稱 | 中性粒細胞殺菌蛋白BP30重組兔單抗 |
| 別 名 | rBPI; BPIFD1; CAP 57; BPI; Bactericidal permeability-increasing protein. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 9D3 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | 54 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human BPI: 120-186 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. SWISS: P17213 Gene ID: 671 殺菌／通透性增加蛋白(Bactericidal／permeability-increasing protein，BPI)是哺乳動物中性粒細胞中存在的一種堿性蛋白，BPI能與革蘭氏陰性菌脂多糖結(jié)合，增加外膜對抗菌藥物的通透性，具有中和內(nèi)毒素和殺滅細菌的生物學作用，在革蘭氏陰性菌感染的治療方面有良好的發(fā)展前景。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with BPI monoclonal antibody, unconjugated (bsm-61275R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
