| 產(chǎn)品編號 | bsm-61302R |
| 英文名稱 | FANCD2 Recombinant Rabbit mAb |
| 中文名稱 | FANCD2重組兔單抗 |
| 別 名 | FA D2; FA4; FAC D2; FACD 2; FACD; FACD2; FAD; FAD2; FANCD 2; FANCD; FANCD2; Fanconi anemia group D2 protein; FLJ23826; Protein FACD2; Type 4 Fanconi pancytopenia. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 17C6 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1:2000,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 164 kDa |
| 檢測分子量 | 166 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human FANCD2: 200-255/1451 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. SWISS: Q9BXW9 Gene ID: 2177 FANCD2是一種在DNA損傷修復過程中起重要作用的蛋白質(zhì)，其作作用在于通過修補DNA損傷或促進有缺陷細胞自殺來預防癌癥，所以低水平的FANCD2會導致DNA損傷，從而誘發(fā)癌癥。 有學者認為:FANCD2是個重要的抵御癌癥蛋白，而香煙會破壞它的制造過程當然還可能包含有其他蛋白，但至少FANCD2是關鍵的主要蛋白，因為含有非常高水平FANCD2的細胞能夠抵御香煙的毒害作用。 & |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with FANCD2 monoclonal antibody, unconjugated (bsm-61302R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
