| 產(chǎn)品編號 | bsm-61489R |
| 英文名稱 | Involucrin Recombinant Rabbit mAb |
| 中文名稱 | 囊包蛋白/內(nèi)披蛋白重組兔單抗 |
| 別 名 | Involucrin; IVL. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10A18 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | 120 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Involucrin: 100-200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. SWISS: P07476 Gene ID: 3713 套膜蛋白又稱內(nèi)披蛋白或囊包蛋白，與毛透明蛋白共同構(gòu)成角質(zhì)細胞包膜支架的蛋白質(zhì)。在角質(zhì)細胞分化時，首先出現(xiàn)在表皮棘層的上層細胞中的標(biāo)志性蛋白質(zhì)，該蛋白在角質(zhì)細胞和其他復(fù)層扁平上皮合成的蛋白。最初存在于細胞質(zhì)中，然后通過轉(zhuǎn)谷氨酰胺酶的作用，轉(zhuǎn)變?yōu)榻宦?lián)的膜蛋白。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Involucrin monoclonal antibody, unconjugated (bsm-61489R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (bsm-61489R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (bsm-61489R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (bsm-61489R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Involucrin Monoclonal Antibody, Unconjugated (bsm-61489R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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