| 產(chǎn)品編號 | bsm-61716R |
| 英文名稱 | IGFBP2 Recombinant Rabbit mAb |
| 中文名稱 | 胰島素樣生長因子結(jié)合蛋白2重組兔單抗 |
| 別 名 | BP2; IBP2; IGF BP53; IGFBP2; IGFBP53. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2D1 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | 35 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IGFBP2: 280-325/325 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. SWISS: P18065 Gene ID: 3485 生長因子和激素（ Growth Factor and Hormones） 胰島素樣生長因子結(jié)合蛋白-2(IGFBP-2)是IGF/IGFBP家族中的一個重要成員,在多種疾病尤其是腫瘤中其表達明顯增高,可能通過調(diào)節(jié)胰島素樣生長因子(insulin-like growth factor,IGF)發(fā)揮作用，促進腫瘤的發(fā)生發(fā)展。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with IGFBP2 monoclonal antibody, unconjugated (bsm-61716R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
