| 產(chǎn)品編號 | bsm-61772R |
| 英文名稱 | LIMK1 Recombinant Rabbit mAb |
| 中文名稱 | LIM結(jié)構(gòu)域激酶1重組兔單抗 |
| 別 名 | LIM kinase; LIMK 1; LIMK. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4D1 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 73 kDa |
| 檢測分子量 | 72 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human LIMK 1: 1-57/647 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. SWISS: P53667 Gene ID: 3984 LIM激酶1(LIMkinase1,LIMK-1)是單絲氨酸蛋白激酶,LIMK-1的活化受多種機制調(diào)控,成為聯(lián)系細胞外刺激與細胞骨架穩(wěn)定性的樞紐,在多種基本的生物學(xué)過程中起重要作用。LIMK-1包含LIM和PDZ蛋白和蛋白相互作用區(qū)、P/S結(jié)構(gòu)域、激酶結(jié)構(gòu)域,在神經(jīng)元中呈高表達。它的主要作用是使肌動蛋白解聚因子cofilin磷酸化和失活,從而導(dǎo)致肌動蛋白細胞骨架的改變。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with LIMK1 monoclonal antibody, unconjugated (bsm-61772R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
