| 產品編號 | bsm-62128R |
| 英文名稱 | DISC1 Recombinant Rabbit mAb |
| 中文名稱 | DISC1重組兔單抗 |
| 別 名 | C1orf136; DISC1; KIAA0457; RP4-730B13.1; SCZD9. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94 kDa |
| 檢測分子量 | 100 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human DISC1: 1-120/854 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Involved in the regulation of multiple aspects of embryonic and adult neurogenesis.Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus.Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. SWISS: Q9NRI5 Gene ID: 27185 DISC-1基因的變異增加了患精神分裂癥的風險。研究人員發(fā)現，抑制老鼠的DISC1活性則改變了動物大腦的發(fā)育，引起了大腦皮層輕微的變異，而在對精神分裂癥病人進行尸檢時在其大腦中見到了此變化。研究人員還發(fā)現將DISC1與對大腦發(fā)育和調節(jié)神經傳遞素水平很重要的分子信號路徑聯系起來，精神病患者的神經傳遞素水平往往是不正常的。 新研究顯示DISC1蛋白質與一個叫PDE4B的酶相互作用，而影響信號傳遞分子cAMP的活性。這個信號傳遞系統(tǒng)過去在其它試驗系統(tǒng)中被發(fā)現與學習、記憶、以及情緒有關， |
| 產品圖片 |
Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC-12 cell lysate. Using DISC1 (bsm-62128R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
