| 產(chǎn)品編號 | bsm-62258R |
| 英文名稱 | Phospho-GSK3 A/B (Tyr279/Tyr216) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化糖原合酶激酶3α/β重組兔單抗 |
| 別 名 | Glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51,47 kDa |
| 檢測分子量 | 51,46 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human GSK3 alpha around the phosphorylation site of Y279: VS-pY-IS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GSK3A a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. SWISS: P49840 Gene ID: 2931 組成性活性蛋白激酶，通過磷酸化和失活糖原合成酶（GYS1或GYS2）、CTNNB1/β連環(huán)蛋白、APC和AXIN1，在激素控制葡萄糖穩(wěn)態(tài)、Wnt信號傳導和轉錄因子及微管的調節(jié)中作為負調節(jié)因子。它的大多數(shù)底物需要啟動磷酸化。通過磷酸化和抑制GYS1活性從而促進糖原合成的胰島素調節(jié)。調節(jié)肝臟的糖原代謝，而不是肌肉。也可能通過調節(jié)轉錄因子的激活來介導胰島素抵抗的發(fā)展。在Wnt信號中，調節(jié)核CTNNB1/β連環(huán)蛋白的水平和轉錄活性。促進淀粉樣前體蛋白（APP）的加工和阿爾茨海默病APP源性淀粉樣斑塊的形成。可能參 |
| 產(chǎn)品圖片 |
Western blot analysis of 293 cell lysate treated with AP. Using Phospho-GSK3 A/B (Tyr279/Tyr216) (bsm-62258R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
