| 產(chǎn)品編號(hào) | bsm-62332R |
| 英文名稱 | Formyl-Histone H2B type 2E (Lys121) Recombinant Rabbit mAb |
| 中文名稱 | |
| 別 名 | Histone H2B |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測(cè)分子量 | 14 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Histone H2B type 2E around the formylation site of K121: VTK(Fo)YT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SWISS: Q16778 Gene ID: 8349 |
| 產(chǎn)品圖片 |
NIH/3T3 (M) cells were treated with or without PMA (100 nM) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Formyl-Histone H2B type 2E (Lys121) monoclonal antibody, unconjugated (bsm-62332R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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