| 產(chǎn)品編號(hào) | bsm-62333R |
| 英文名稱 | PLAA Recombinant Rabbit mAb |
| 中文名稱 | 磷脂酶A2激活蛋白重組兔單抗 |
| 別 名 | Glycerophosphatase; PLA2P; Plaa; PLAP. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 87 kDa |
| 檢測(cè)分子量 | 75 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PLAP: 750-795/795 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Plays a role in protein ubiquitination, sorting and degradation through its association with VCP.Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling.May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. SWISS: Q9Y263 Gene ID: 9373 磷脂酶A2激活蛋白能激活磷脂酶A2，是類花生酸形成的一個(gè)重要中介體。作為促炎癥反應(yīng)劑，PLAP存在于類風(fēng)濕性關(guān)節(jié)炎患者的炎癥組織和滑膜液，因而被認(rèn)為在炎癥性疾病調(diào)控中發(fā)揮重要作用。IL-1β和TNF-α能刺激PLAP的形成。PLAP作為睪丸精原細(xì)胞瘤的標(biāo)志物。 |
| 產(chǎn)品圖片 |
Western blot analysis of MCF7 cell lysate. Using PLAA (bsm-62333R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PLAA antibody (bsm-62333R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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