| 產(chǎn)品編號 | bsm-62434R |
| 英文名稱 | TERT Recombinant Rabbit mAb |
| 中文名稱 | 端粒酶反轉(zhuǎn)錄酶重組兔單抗 |
| 別 名 | TCS1; HEST2; EST2; TRT; Telomerase reverse transcriptase; Telomerase-associated protein 2; Telomerase catalytic subunit. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 127 kDa |
| 檢測分子量 | 127 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TERT: 1000-1132 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. SWISS: O14746 Gene ID: 7015 端粒酶逆轉(zhuǎn)錄酶hTERT是構成端粒酶的組分之一，是端粒酶活性的必需和限速成分，水平?jīng)Q定細胞端粒酶的活性。抑制hTERT可降低端粒酶的活性，從而抑制瘤細胞生長。目前對hTERT的研究已成為端粒酶研究的熱點問題，已發(fā)現(xiàn)TERT蛋白表達在腫瘤診斷中有重要意義，并制備了hTERT抗體及應用核酶技術等來抑制hTERT蛋白的表達，抑制端粒酶活性，從而抑制腫瘤的生長。 端粒反轉(zhuǎn)錄酶又稱端粒酶催化亞單位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associa |
| 產(chǎn)品圖片 |
Western blot analysis of Hela cells lysate. Using TERT (bsm-62434R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
