| 產(chǎn)品編號(hào) | bs-0378R |
| 英文名稱(chēng) | Runx3 Rabbit pAb |
| 中文名稱(chēng) | Runx3抗體 |
| 別 名 | RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3(CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit(PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; |
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Specific References (2) | bs-0378R has been referenced in 2 publications.
[IF=3.48] He, Jian?\Feng, et al. "xpression of RUNX3 in salivary adenoid cystic carcinoma: Implications for tumor progression and prognosis." Cancer science 99.7 (2008): 1334-1340.. IHC-P ; Human.
[IF=2.35] Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017). WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Runx3: 151-250/415 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Subunit: Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Subcellular Location: Nucleus. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Post-translational modifications: Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. Similarity: Contains 1 Runt domain. SWISS: Q13761 Gene ID: 864 Database links: Entrez Gene: 864 Human Omim: 600210 Human SwissProt: Q13761 Human Unigene: 170019 Human 新型抑癌基因/一種新發(fā)現(xiàn)的抑癌基因。經(jīng)研究發(fā)現(xiàn),在人類(lèi)胃癌細(xì)胞系和胃癌組織中普遍存在Runx3基因表達(dá)的缺失或下調(diào),該蛋白與胃癌的發(fā)生、發(fā)展密切相關(guān)。 RUNX3基因被認(rèn)為是一種新發(fā)現(xiàn)的抑癌基因,對(duì)胃癌細(xì)胞的生長(zhǎng)有明顯的抑制作用在胃黏膜上皮細(xì)胞調(diào)控、對(duì)脊神經(jīng)節(jié)的神經(jīng)發(fā)育和T細(xì)胞分化中都發(fā)揮重要作用。 隨著研究的不斷深入，Runx3蛋白有望成為胃癌診斷的一個(gè)新型生物學(xué)標(biāo)志物和基因治療靶點(diǎn)。 |
| 產(chǎn)品圖片 |
Sample: A549 Cell Lysate at 30 ug
Primary: Anti- Runx3 (bs-0378R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample: Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- Runx3 (bs-0378R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated(bs-0378R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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