| 產(chǎn)品編號(hào) | bs-11755R |
| 英文名稱(chēng) | SRSF7 Rabbit pAb |
| 中文名稱(chēng) | 絲氨酸/蘇氨酸蛋白激酶SRPK7抗體 |
| 別 名 | 21 GARP; 21 glutamic acid rich protein; 21-GARP; SH3 domain binding glutamic acid rich protein; SH3BGR; SH3BGR protein; Serine/arginine-rich splicing factor 7; SRSF7_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Cow,Zebrafish,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRSF7: 2-70/238 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns, and they are required for accurate splice site recognition and the control of alternative splicing (1). Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF (2). The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, and they are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites (3,4). The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport (5). The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites (6). Function: Splicing factor, arginine/serine rich 7 is required for pre mRNA splicing. Can also modulate alternative splicing in vitro. Subunit: Found in large molecular weight complexes containingCCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interactswith CCNL2 and CPSF6. Subcellular Location: Nuclear Tissue Specificity: Brain, liver, kidney and lung. Post-translational modifications: Extensively phosphorylated on serine residues in the RSdomain. Similarity: Belongs to the splicing factor SR family. Contains 1 CCHC-type zinc finger. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q16629 Gene ID: 6432 Database links: Entrez Gene: 6432 Human Omim: 600572 Human SwissProt: Q16629 Human Unigene: 309090 Human |
| 產(chǎn)品圖片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SFRS7 Polyclonal Antibody, Unconjugated(bs-11755R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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