| 產(chǎn)品編號 | bs-12987R |
| 英文名稱 | DCPS Rabbit pAb |
| 中文名稱 | 清道夫脫帽酶/熱休克蛋白1抗體 |
| 別 名 | 1700001E16Rik; AA408441; DCPS; DCPS_HUMAN; DCS 1; DCS-1; DCS1; Decapping enzyme scavenger; heat shock-like protein 1; Hint 5; Hint related 7meGMP directed hydrolase; HINT-5; Hint-related 7meGMP-directed hydrolase; HINT5; Histidine triad protein member 5; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; HSL1; HSPC015; m7GpppX diphosphatase; MGC124934; mRNA decapping enzyme; OTTMUSP00000042220; Scavenger mRNA decapping enzyme DcpS; Scavenger mRNA-decapping enzyme DcpS. |
| 研究領(lǐng)域 | 細(xì)胞生物 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DCPS: 101-200/337 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Eukaryotic cells primarily utilize exoribonucleases and decapping enzymes to degrade their mRNA. DcpS is a scavenger pyrophosphatase that hydrolyzes the residual cap structure following 3' to 5' decay of an mRNA. Following mRNA degradation DcpS releases N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. The central histidine within the DcpS HIT motif is critical for decapping activity and defines the HIT motif as a new mRNA decapping domain, making DcpS the first member of the HIT family of proteins with a defined biological function. HIT proteins are homodimeric and contain two conserved 100-amino-acid HIT fold domains with independent active sites that are each sufficient to bind and hydrolyze cognate substrates. Function: Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides. Subunit: Homodimer. Associates with components of the exosome multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts with NDOR1. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Detected in liver, brain, kidney, testis and prostate. Similarity: Belongs to the HIT family. SWISS: Q96C86 Gene ID: 28960 Database links: Entrez Gene: 28960 Human Omim: 610534 Human SwissProt: Q96C86 Human Unigene: 504249 Human |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DCPS Polyclonal Antibody, Unconjugated(bs-12987R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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