| 產(chǎn)品編號 | bs-7360R |
| 英文名稱 | Topoisomerase III Rabbit pAb |
| 中文名稱 | DNA拓普西異構(gòu)酶Topo IIIα抗體 |
| 別 名 | DNA topoisomerase 3 alpha; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; TOP 3; TOP3; TOP3A; TOP3A_HUMAN; topoisomerase(DNA) III alpha; topoisomerase(DNA) III; topoisomerase III alpha; Topo-IIIα. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 112 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Topoisomerase III: 271-370/1001 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. [provided by RefSeq, Jul 2008] Function: Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Tissue Specificity: High expression is found in testis, heart, skeletal muscle and pancreas. Similarity: Belongs to the prokaryotic type I/III topoisomerase family. Contains 1 Toprim domain. SWISS: Q13472 Gene ID: 7156 Database links: Entrez Gene: 7156 Human Omim: 601243 Human SwissProt: Q13472 Human Unigene: 592115 Human |
| 產(chǎn)品圖片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TOP3 Polyclonal Antibody, Unconjugated(bs-7360R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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