| 產(chǎn)品編號 | bs-16166R |
| 英文名稱 | FOXJ2 Rabbit pAb |
| 中文名稱 | FOXJ2蛋白抗體 |
| 別 名 | FHX; Fork head homologous X; Forkhead box J2; Forkhead box protein J2; FOXJ2; FOXJ2_HUMAN. |
| 研究領域 | 細胞生物 免疫學 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOXJ2: 111-210/574 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
FOXJ2, Forkhead box protein J2, is a 574 amino acid protein encoded by the human gene FOXJ2. FOXJ2 is a novel forkhead factor, belonging to the forkhead family, with a dual DNA binding specificity. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. In the testis, FOXJ2 is expressed from pachytene spermatocytes to round spermatids, but not in spermatogonia. In addition to the germ lineage, only Sertoli cells of the testis showed expression of FOXJ2. In the ovary, only granulosa cells of the follicles express the factor. Neither mature spermatozoa nor oocytes have been found to express FOXJ2. However, both the trophectoderm (TE) and the inner cell mass (ICM) cell layers of the blastocyst express FOXJ2. Function: Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: Q9P0K8 Gene ID: 55810 Database links: Entrez Gene: 55810 Human Entrez Gene: 60611 Mouse SwissProt: Q9P0K8 Human SwissProt: Q9ES18 Mouse Unigene: 120844 Human Unigene: 87142 Mouse |
| 產(chǎn)品圖片 |
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-FOXJ2 (bs-16166R) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
