| 產品編號 | bs-16176R |
| 英文名稱 | FOXR2 Rabbit pAb |
| 中文名稱 | 叉頭蛋白R2抗體 |
| 別 名 | Forkhead box protein N6; Forkhead box protein R2; FOXN6; FOXR2_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOXR2: 51-150/311 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The Forkhead-box (FOX) genes comprise a superfamily of at least 43 members that express proteins which are involved in transcriptional regulation and may be associated with the pathogenesis of various cancers. FOXR2 (Forkhead box protein R2), also known as FOXN6 (Forkhead box protein N6), is a 311 amino acid nuclear protein that belongs to the FOX family and contains one fork-head DNA-binding domain. Like other members of the FOX family, FOXR2 is not abundantly expressed in normal tissues but is found at very high levels in breast cancer and primary cancer cell lines and is thought to act as a transcription factor. Through chromosomal aberrations such as retroviral integration, gene amplification or translocation, FOXR2 may be involved the development of certain invasive carcinomas. Function: Forkhead-box (FOX) transcription factors are implicated in carcinogenesis through gene amplification, retroviral integration, or chromosomal translocation. Human FOXR2 mRNA was expressed in breast cancer cell line and primary breast cancer. Subcellular Location: Nuclear Tissue Specificity: Expressed in breast cancer cell lines and primary cancer. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: Q6PJQ5 Gene ID: 139628 Database links: Entrez Gene: 139628 Human SwissProt: Q6PJQ5 Human Unigene: 364260 Human |
| 產品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- FOXR2 (bs-16176R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BrdU) polyclonal Antibody, Unconjugated (bsm-0917M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
