| 產(chǎn)品編號 | bs-16328R |
| 英文名稱 | GRWD1 Rabbit pAb |
| 中文名稱 | GRWD1蛋白抗體 |
| 別 名 | CDW4; CUL4 and DDB1 associated WDR protein 4; Glutamate rich WD repeat containing 1; Glutamate rich WD repeat containing protein 1; Glutamate rich WD repeat protein GRWD; Glutamate-rich WD repeat-containing protein 1; GRWD 1; GRWD; grwd1; GRWD1_HUMAN; KIAA1942; Regulator of ribosome biogenesis 1 homolog; RRB 1; RRB1; WD repeat domain 28; WDR 28; WDR28. |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRWD1: 301-400/446 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a glutamate-rich protein that contains five WD-repeat motifs. The encoded protein may play a critical role in ribosome biogenesis and may also play a role in histone methylation through interactions with CUL4-DDB1 ubiquitin E3 ligase. [provided by RefSeq, Feb 2012] Subunit: Interacts with METTL18. Subcellular Location: Nucleus, nucleolus. Similarity: Contains 5 WD repeats. SWISS: Q9BQ67 Gene ID: 83743 Database links: Entrez Gene: 468939 Chimpanzee Entrez Gene: 83743 Human Entrez Gene: 101144842 Gorilla Entrez Gene: 100733019 Guinea pig Entrez Gene: 100051020 Horse Entrez Gene: 100386385 Marmoset (common) Entrez Gene: 100441405 Orangutan Entrez Gene: 717966 Rhesus monkey Omim: 610597 Human SwissProt: Q9BQ67 Human Unigene: 400625 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRWD1) Polyclonal Antibody, Unconjugated (bs-16328R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
