| 產(chǎn)品編號 | bs-17323R |
| 英文名稱 | TopBP1 Rabbit pAb |
| 中文名稱 | DNA拓撲異構(gòu)酶2結(jié)合蛋白1抗體 |
| 別 名 | DNA topoisomerase 2 binding protein 1; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II binding protein 1; DNA topoisomerase II binding protein; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; DNA topoisomerase IIbeta binding protein 1; Hypothetical protein KIAA0259 [Fragment]; KIAA0259; TOP2BP1; TOPB1_HUMAN; TOPBP 1; TopBP1; Topoisomerase(DNA) II binding protein 1; Topoisomerase II binding protein 1. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 171 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TopBP1: 851-950/1522 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Subcellular Location: Nucleus. Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Tissue Specificity: Highly expressed in heart, brain, placenta, lung and kidney. Post-translational modifications: Phosphorylated on serine and threonine residues in response to X-ray irradiation. Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Similarity: Contains 8 BRCT domains. SWISS: Q92547 Gene ID: 11073 Database links: Entrez Gene: 11073 Human Entrez Gene: 235559 Mouse Omim: 607760 Human SwissProt: Q92547 Human SwissProt: Q6ZQF0 Mouse Unigene: 593379 Human Unigene: 259893 Mouse |
| 產(chǎn)品圖片 |
Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti-TopBP1 (bs-17323R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 171 kD
Observed band size: 171 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
