產(chǎn)品編號 | bs-18106R |
英文名稱 | hUPF2/RENT2 Rabbit pAb |
中文名稱 | hUPF2蛋白抗體 |
別 名 | DKFZp434D222; hUpf2; KIAA1408; MGC138834; MGC138835; Nonsense mRNA reducing factor 2; Regulator of nonsense transcripts 2; RENT2; RENT2_HUMAN; Smg 3; Smg 3 homolog nonsense mediated mRNA decay factor; Up-frameshift suppressor 2 homolog; UPF2; UPF2 regulator of nonsense transcripts homolog; UROC1; Urocanate hydratase; Yeast Upf2p homolog. |
研究領(lǐng)域 | 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog,Ferret,Gorilla,Opossum,Platypus) |
產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 148 kDa |
檢測分子量 | |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human hUPF2/RENT2: 951-1050/1272 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008] Function: Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA. Subcellular Location: Cytoplasm > perinuclear region. Tissue Specificity: Ubiquitous. Similarity: Contains 3 MIF4G domains. SWISS: Q9HAU5 Gene ID: 26019 Database links: Entrez Gene: 450304 Chimpanzee Entrez Gene: 100069342 Horse Entrez Gene: 26019 Human Entrez Gene: 326622 Mouse Omim: 605529 Human SwissProt: Q9HAU5 Human Unigene: 370689 Human Unigene: 610110 Human Unigene: 31102 Mouse |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hUPF2) Polyclonal Antibody, Unconjugated (bs-18106R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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