| 產(chǎn)品編號 | bs-18856R |
| 英文名稱 | RREB1 Rabbit pAb |
| 中文名稱 | RREB1蛋白抗體 |
| 別 名 | FINB; Finger protein in nuclear bodies; Hindsight homolog; HNT; LZ321; Raf responsive zinc finger protein LZ321; Raf-responsive zinc finger protein LZ321; RAS responsive element binding protein 1; Ras-responsive element-binding protein 1; RREB 1; RREB-1; RREB1_HUMAN; Zep 1; Zep-1; Zep1; Zinc finger motif enhancer binding protein 1; Zinc finger motif enhancer-binding protein 1. |
| 研究領域 | 細胞生物 信號轉導 轉錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 181 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RREB1: 1-100/1687 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] Function: Transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. Subcellular Location: Nucleus speckle. Tissue Specificity: Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not found in the brain. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 15 C2H2-type zinc fingers. SWISS: Q92766 Gene ID: 6239 Database links: Entrez Gene: 6239 Human Entrez Gene: 68750 Mouse Omim: 602209 Human SwissProt: Q92766 Human SwissProt: Q3UH06 Mouse Unigene: 298248 Human Unigene: 132634 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RREB1) Polyclonal Antibody, Unconjugated (bs-18856R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
