| 產(chǎn)品編號(hào) | bs-19024R |
| 英文名稱 | NARS2 Rabbit pAb |
| 中文名稱 | 天冬氨酰tRNA連接酶2抗體 |
| 別 名 | AI875199; AsnRS; Asparagine tRNA ligase 2; Asparagine tRNA ligase 2 mitochondrial; Asparagine tRNA ligase; Asparaginyl tRNA synthetase 2; Asparaginyl tRNA synthetase 2 mitochondrial; FLJ23441; MGC125113; MGC41336; NARS 2; Probable asparaginyl tRNA synthetase; Probable asparaginyl tRNA synthetase mitochondrial; RGD1305145; SLM5. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NARS2: 101-200/477 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SLM5 (mitochondrial asparaginyl-tRNA synthetase, Asparagine-tRNA ligase) is a mitochondrial protein encoded by the human gene NARS2. AsnRS belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl tRNA synthetases (aaRS) are enzymes that catalyze the esterification of a specific amino acid or its precursor to its compatible cognate tRNA to form an aminoacyl-tRNA. The synthetase first binds ATP and the corresponding amino acid or its precursor to form an aminoacyl-adenylate and release inorganic pyrophosphate (PPi). The adenylate-aaRS complex then binds the appropriate tRNA molecule, and the amino acid is transferred from the aa-AMP to either the 2'- or 3'-OH of the last tRNA base (A76) at the 3'-end. Some synthetases also mediate a proofreading reaction to ensure high fidelity of tRNA charging; if the tRNA is found to be improperly charged, the aminoacyl-tRNA bond is hydrolyzed. SLM5 acts to attach asparagine residues to its cognate tRNA. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. SWISS: Q96I59 Gene ID: 79731 Database links: Entrez Gene: 79731 Human SwissProt: Q96I59 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARS2) Polyclonal Antibody, Unconjugated (bs-19024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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