| 產品編號 | bs-8845R |
| 英文名稱 | Synaptophysin Rabbit pAb |
| 中文名稱 | 突觸小泡蛋白P38抗體 |
| 別 名 | Major synaptic vesicle protein P38; MRXSYP; Syn p38; SYP; SYPH; SYPH_HUMAN; SypI. |
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Specific References (3) | bs-8845R has been referenced in 3 publications.
[IF=5.702] Yue-Ming Zhang. et al. Long-term environmental enrichment overcomes depression, learning, and memory impairment in elderly CD-1 mice with maternal sleep deprivation exposure. FRONT AGING NEUROSCI. 2023; 15: 1177250 WB ; Mouse.
[IF=2.923] Heng Yang. et al. miR-665 overexpression inhibits the apoptosis of luteal cells in small ruminants suppressing HPGDS. THERIOGENOLOGY. 2023 Aug;206:40 ICC ; Sheep.
[IF=2.889] Bin Liu. et al. Nicotinamide Improves Cognitive Function in Mice With Chronic Cerebral Hypoperfusion. Front Neurol. 2021; 12: 596641 WB,IHC ; Mouse.
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| 研究領域 | 細胞生物 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptophysin: 151-250/313 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011] Function: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Subcellular Location: Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Tissue Specificity: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Post-translational modifications: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. DISEASE: Defects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Similarity: Belongs to the synaptophysin/synaptobrevin family. Contains 1 MARVEL domain. SWISS: P08247 Gene ID: 6855 Database links: Entrez Gene: 6855 Human Entrez Gene: 20977 Mouse Omim: 313475 Human SwissProt: P08247 Human SwissProt: Q62277 Mouse Unigene: 632804 Human Unigene: 223674 Mouse Unigene: 11067 Rat |
| 產品圖片 |
Sample:
Cerebrum (mouse) Lysate at 40 ug
Primary: Anti- Synaptophysin (bs-8845R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (bs-8845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (bs-8845R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (bs-8845R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
