| 產(chǎn)品編號(hào) | bsm-33184M |
| 英文名稱 | AmCyan Mouse mAb |
| 中文名稱 | AmCyan藍(lán)色熒光蛋白單克隆抗體 |
| 別 名 | AmCyan Tag; AmCyan-Tag; 野生型天竺葵青色熒光蛋白; 藍(lán)色熒光蛋白AmCyan標(biāo)簽; 藍(lán)色熒光蛋白標(biāo)簽; |
| 產(chǎn)品類型 | 標(biāo)簽抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 5F9 |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant AmCyan |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33184P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- AmCyan-Tag (bsm-33184M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
|
