| 產(chǎn)品編號 | bsm-60444R |
| 英文名稱 | ABCE1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 別 名 | ATP-binding cassette sub-family F(GCN20) member 1; Abc50; GCN20; AU041969; D17Wsu166e; Abcf1; ABC27; ATP binding cassette 50(TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; EST123147; TNF alpha stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667; ABCF1_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | A12F4 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human ABCE1: 574-599 |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 共翻譯質(zhì)量控制因子參與了不通過衰變（NGD）途徑。與PELO和HBS1L一起，是80S核糖體形成48S復(fù)合物和空位80S核糖體解離所必需的。與PELO和HBS1L一起識別停滯的核糖體并促進組裝在不間斷mRNAs上的延伸復(fù)合物的解離，這觸發(fā)了mRNA的核內(nèi)裂解，這是一種釋放非功能性核糖體和降解受損mRNAs的機制，是No-Go衰變（NGD）途徑的一部分。在mRNA周轉(zhuǎn)的調(diào)控中起作用，在線粒體外膜定位mRNA翻譯的質(zhì)量控制中起作用。作為PINK1調(diào)控信號的一部分，在線粒體損傷時被CNOT4泛素化；這種修飾產(chǎn)生多泛素信號，將自噬受體招募到線粒體外膜并啟動有絲分裂吞噬。RNASEL特異性蛋白抑制劑，拮抗2-5A（5'-磷酸化2'，5'-連接的寡腺苷酸）與RNASEL的結(jié)合。干擾素調(diào)節(jié)的2-5A/RNASEL途徑抗病毒作用的負調(diào)節(jié)因子。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ABCE1 monoclonal antibody, unconjugated (bsm-60444R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (bsm-60444R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
