| 產(chǎn)品編號(hào) | bsm-60214M |
| 英文名稱 | Histone H4 (NT) Mouse mAb |
| 中文名稱 | |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | D12G5 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Monkey) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 核小體由四個(gè)核心組蛋白（H2A、H2B、H3和H4）組成，是染色質(zhì)的主要組成部分。核小體將DNA包裹并壓縮成染色質(zhì)，限制DNA進(jìn)入細(xì)胞機(jī)制。組蛋白H4是參與真核細(xì)胞染色質(zhì)結(jié)構(gòu)的五種主要組蛋白之一。H4具有一個(gè)主要的球狀結(jié)構(gòu)域和一個(gè)長(zhǎng)的N末端尾巴，它參與了“串珠”組織的核小體結(jié)構(gòu)。組蛋白是高度翻譯后修飾的。共價(jià)鍵合修飾包括N端尾部的乙?；图谆?。這些修飾可能改變位于與其親本組蛋白八聚體相關(guān)的DNA上的基因的表達(dá)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: MCF-7, BRL, N2a, COS-7, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60214M
|
