| 產(chǎn)品編號 | bs-25393R |
| 英文名稱 | POLK Rabbit pAb |
| 中文名稱 | DNA聚合酶κ/DNA pol κ抗體 |
| 別 名 | DINP; POLQ; DINB1; DNA polymerase kappa; DINB protein; POLK_HUMAN. |
| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POLK: 1-100/870 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016] Gene ID: 51426 Database links: Entrez Gene: 51426 Human SwissProt: Q9UBT6 human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Prostate tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human MOLT4 cell lysates
Primary: Anti-POLK (bs-25393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 100 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human POLK protein, His Tag (Cat. bs-25393P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human POLK Antibody, the EC50 is 199.8 ng/mL.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
