| 產(chǎn)品編號 | bs-17142R-BF488 |
| 英文名稱 | Rabbit Anti-TRMT1/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的TRMT1蛋白抗體 |
| 別 名 | TRM1; 2)G26)dimethyltransferase; 2-dimethylguanosine-26 methyltransferase; FLJ20244; N(2) N(2) dimethylguanosine tRNA methyltransferase; N(2)-N(2)) methyltransferase; TRM 1; TRM1 tRNA methyltransferase 1 homolog; TRM1_HUMAN; TRMT 1; Trmt1; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA 2 2 dimethylguanosine 26 methyltransferase; tRNA 2; tRNA(guanine 26 N(2) N(2)) methyltransferase; tRNA(guanine-26; tRNA(m(2 2)G26)dimethyltransferase; tRNA(m(2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 72kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames. N(2),N(2)-dimethylguanosine tRNA methyltransferase, also known as TRMT1 or tRNA(guanine-26,N(2)-N(2)) methyltransferase, is a 659 amino acid enzyme that is responsible for tRNA modifications in eukaryotes. Using S-adenosyl-L-methionine as a methyl donor, TRMT1 dimethylates a single guanine residue at position 26 of tRNA. TRMT1, which was initially identified in yeast and C. elegans, has a 26% and 31% sequence identity to its yeast and C. elegans homologs, respectively. There are two isoforms of TRMT1 produced by alternative splicing events. The TRMT1 gene maps to chromosome 19p13.13 and mutations in this gene lead to abrogated enzyme activity and a decrease in protein levels. Function: Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups. Similarity: Contains 1 C3H1-type zinc finger. Database links: Entrez Gene: 55621 Human Entrez Gene: 212528 Mouse Omim: 611669 Human SwissProt: Q9NXH9 Human SwissProt: Q3TX08 Mouse Unigene: 515169 Human Unigene: 275720 Mouse Unigene: 17073 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
